Journal: Scientific Reports
Article Title: Heterozygous interferon signaling deficient mice as animal models for Chikungunya virus infection in the heart
doi: 10.1038/s41598-025-02191-9
Figure Lengend Snippet: Increase the expression of cytokines, chemokines, and neutrophil infiltration in the hearts of CHIKV-infected ifnag +/− mice. WT, ifnar1 +/− , and ifnag +/− mice were infected with CHIKV (1 × 10 5 PFU) via footpad, and the hearts were collected on D1 p.i. after perfusion. ( A ) The RT-qPCR analysis of the expression of Tnf-α , Il-6 , Il-1β , Cxcl1 , Cxcl2 , Cxcl10 , and Cxcl12 in the hearts. ( B -left) Immunohistochemistry of the heart sections of WT, ifnar1 +/− , and ifnag +/− mice stained with DAPI (blue, for nuclei), Alexa Fluor 488 (green, for anti-Chikungunya virus antibody), Alexa Fluor 568 (yellow, for leukocyte marker CD45), and observed under a confocal microscope at 20× magnification. Colocalizations of CHIKV E1 and CD45 are marked by orange arrows. The scale bars of all images are 25 μm. ( B -right) Quantification of CD45 + cells in a bright field of confocal images. ( C -left) Flow cytometry results of the neutrophil infiltration in the hearts of WT, ifnar1 +/− , and ifnag +/− mice. The infiltrated neutrophils are defined as CD45 + CD11b + Ly6G + cells. ( C -right) Quantification of the infiltrated neutrophils in the hearts. The data presented are from at least two independent experiments performed in triplicate. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test. Statistical significance is indicated as * p < 0.05 and *** p < 0.001.
Article Snippet: The heart-infiltrated leukocytes were probed with antibodies CD45 (PE-Cy7-conjugated, eBioscience), CD11b (APC-conjugated, BD Biosciences), and Ly6G (APC Cy7-conjugated, Invitrogen).
Techniques: Expressing, Infection, Quantitative RT-PCR, Immunohistochemistry, Staining, Virus, Marker, Microscopy, Flow Cytometry
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